Fibre-optic ultra-micro cell for UV/Vis analysis
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- In detail
The TrayCell is a fibre-optic ultra-micro cell designed for the UV/Vis analysis of DNA/RNA and proteins.
The Hellma TrayCell is designed for measurements e.g. of DNA/RNA or protein samples and enables highly accurate analysis of extremely small samples with remarkable reproducibility. The dimensions of the TrayCell are equivalent to a standard cuvette in order to work in most spectrophotometers.
Using the 1 mm or 0.2 mm cap creates a defined optical light path of 1 mm and 0.2 mm respectively. This generates virtual dilution factors of 1:10 or 1:50 in comparison to a measurement with a standard 10 mm cuvette. This feature saves time and avoids dilution errors. If desired, samples can be retrieved after the measurement for further processing. The required sample volume for the 1 mm cap is 3 μl to 5 μl and for the 0.2 mm cap 0.7 μl to 4 μl.
With the Hellma TrayCell the average dynamic range for dsDNA is between 2 ng/μl and 5000 ng/μl. The mean dynamic range depends strongly on the type of photometer used.
Due to the integrated beam deflection and the use of fibre-optic cables it is possible to measure the sample directly on the surface of the optical window. The cap with mirror provides a well-defined optical light path and prevents the sample from drying up. The measurement remains reproducible because the sample will not be enriched by evaporation of the solvent.